MORPHOMETRIC ANALYSIS AND VIRULENCE OF ISOLATES OF TOXOPLASMA GONDII RECOVERED FROM PREGNANT AND NEWBORN MICE IN GOIÂNIA-GO

  • Marcos Gontijo da Silva Centro Universitário Unirg
  • Fuad Moares Ibrahim Associate Professor of the Medicine course at the University Center UNIRG
  • Gustavo José Von Glehn dos Santos Associate Professor of the Medicine course at the University Center UNIRG.
  • Omar Franklin Molina Adjunct professor of the Physical Education course at the University Center UNIRG
  • João Bartholomeu Neto Adjunct professor of the Physical Education course at the University Center UNIRG.
  • Erica Eugênio Lourenço Gontijo Associate Professor of the Pharmacy Course at UNIRG University Center.
  • Ana Maria de Castro Associate Professor at the Federal University of Goias

Abstract

Studies in Toxoplasma gondii carried out in newborns are interesting and relevant as they induce serious lesions in newborns when they are not properly treated. Three isolates of Toxoplasma gondii in newborns and the RH strain were assessed regarding pathogenicity and virulence in Balb-c albino mice. Tachizoite suspension used in experimental tests was obtained through punction or lavage from the peritoneal cavity in mice presenting with ascites. Each sample was inoculated in groups of four mice with inoculates of 101, 102, 103, 104, 105, 106 alive tachyzoites, via intra peritoneum. We found that isolates 15, 20 and RH strain behaved lethally to mice. Isolate-3 behaved lethally in all mice up to 105 tachyzoites. Death of all animals that presented acute infection occurred 5-10 days following inoculation.  Isolate-15, isolate-20 and RH strain presented a greater diameter and minor areas as compared with isolate-3. We found that the most virulent isolate also demonstrated greater area and diameter and these findings reinforces the hypotheses that besides behavioral differences and genetic variations, morphological variations also exist when different strains of T. gondii are evaluated.

Author Biography

Marcos Gontijo da Silva, Centro Universitário Unirg
Doutor em Medicina tropical pela Universidade Federal de Goiás (2014), Mestre em Medicina Tropical pela Universidade Federal de Goiás (2006), Possui graduação em Biomedicina pela Universidade Federal de Goiás (2003), graduação em Ciências Biológicas pela Universidade Federal de Goiás (2001). Atualmente é Pró-Reitor de Pesquisa e Pós-Graduação e professor titular de parasitologia do Centro Universitário UNIRG em Gurupi,Tocantins. Tem experiência na área de Parasitologia, com ênfase em Protozoologia Humana, atuando principalmente nos seguintes temas: Epidemiologia, clínica e diagnóstico da toxoplasmose gestacional e congênita.
Published
2018-05-21