Diversity and Biotechnological Potential of Endophytic Fungi Isolated in Pantanal

Abstract

The Pantanal is a natural heritage site that is home to various species, including endophytic fungi that produce bioactive compounds. This study aimed to isolate and phenotypically characterize endophytic fungi from plants in the Pantanal region of Mato Grosso do Sul, evaluating the compounds produced. For isolation, samples were sanitized and plant fragments were grown on Potato Dextrose Agar medium, incubated at 30ºC. After growth, colonies were picked to obtain pure cultures. The isolates were visually characterized on plates, both on the front and reverse sides. To verify pigment production, the fungi were grown in Potato Dextrose medium and incubated at 5ºC after growth at 30ºC. Enzymatic activity was assessed by the formation of halos in specific media for each enzyme. In total, 16 fungi were isolated, of which 5 presented distinct phenotypic characteristics, and two produced pigments in pink and green colors. The isolates demonstrated enzymatic activity for cellulase, indicating biotechnological potential. This study highlights the importance of preserving the Pantanal, which functions as a natural reservoir of resources with potential for various applications.