BAP e tempo de cultivo na multiplicação in vitro de Khaya grandifoliola

João Victor Silveira; Natane  Amaral Miranda

João Victor Silveira Universidade Federal de Viçosa https://orcid.org/0009-0002-7331-4812
Natane Amaral Miranda Universidade Federal Rural do Rio de Janeiro https://orcid.org/0000-0001-8197-5421

Abstract

Khaya grandifoliola, popularly known as African mahogany, stands out as an important forest species, widely used in the furniture and construction sectors. The present work aimed to evaluate the influence of BAP and cultivation time on the in vitro multiplication of Khaya grandifoliola, using nodal segments. Concentrations of 0, 0.5; 1.0; 2.0 and 4.0 mg L^-1 of BAP added to the nutrient medium, with the plants being evaluated from 25 to 60 days of cultivation, at intervals of 5 days. The influence of the growth regulator and cultivation time on the in vitro development of plants was observed. The addition of BAP to the nutrient medium provided an increase in the number of buds, as well as callus production, but was not sufficient for the generation of shoots, demonstrating its potential for use in other cell multiplication methods of the species. After 25 days of in vitro cultivation of Khaya grandifoliola, better tissue vigor was observed, indicating a rapid response to hormonal stimulation. For in vitro multiplication of the species, it is recommended to investigate other growth regulators available on the market, in addition to continuing studies in the area.

Author Biographies

João Victor Silveira, Universidade Federal de Viçosa

Engenheiro Florestal pela Universidade Federal Rural do Rio de Janeiro (UFRRJ) e Mestrando em Genética e Melhoramento pela Universidade Federal de Viçosa (UFV). Possui enfoque na área da propagação vegetativa, genética, melhoramento e biotecnologia vegetal.

Natane Amaral Miranda, Universidade Federal Rural do Rio de Janeiro

Possui graduação em Engenharia Florestal (2013) e mestrado em Ciência Florestal (2015) pela Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM). Doutora em Ciência Florestal (2018) pela Universidade Federal de Viçosa (UFV). Atua na área de Propagação de plantas, Melhoramento e Biotecnologia Florestal. Atualmente é professora Adjunta na Universidade Federal Rural do Rio de Janeiro.